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An efficient 3D NMR technique for correlating the proton and15N backbone amide resonances with the α-carbon of the preceding residue in uniformly15N/13C enriched proteins

✍ Scribed by Ad Bax; Mitsuhiko Ikura


Publisher
Springer Netherlands
Year
1991
Tongue
English
Weight
486 KB
Volume
1
Category
Article
ISSN
0925-2738

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✍ Rüdiger Weisemann; Frank Löhr; Heinz Rüterjans 📂 Article 📅 1994 🏛 Springer Netherlands 🌐 English ⚖ 489 KB

A 3D triple resonance experiment has been designed to provide intraresidual and sequential correlations between amide nitrogens and c~-carbons in uniformly 13C/15N-labeled proteins. In-phase 13C~ magnetization is transferred to the aliphatic side-chain protons via the side-chain carbons using a CC-T