✦ LIBER ✦

Measurement of lactate accumulation by in Vivo proton NMR spectroscopy during global cerebral ischemia in rats

✍ Scribed by T. L. Richards; M. A. Keniry; P. R. Weinstein; B. M. Pereira; B. T. Andrews; E. J. Murphy; T. L. James

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 295 KB
Volume: 5
Category: Article
ISSN: 0740-3194
DOI: 10.1002/mrm.1910050406

No coin nor oath required. For personal study only.

✦ Synopsis

In vivo 'H NMR spectral editing techniques were used to monitor cerebral lactate production during remotely controlled temporary forebrain ischemia in rats. The lactate/Nacetylaspartate ( N U ) ratio correlated with suMval after ischemia and subsequent reperfusion. The lactate/NAA ratio that predicted death after ischemia in rats was estimated to be approximately 1.3. o 1987 Academic press, Inc.

📜 SIMILAR VOLUMES

Quantitative t1ρ NMR spectroscopy of rat

Quantitative t1ρ NMR spectroscopy of rat cerebral metabolites in vivo: Effects of global ischemia

✍ Mikko I. Kettunen; Olli H.J. Gröhn; Risto A. Kauppinen 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 111 KB

## Abstract The NMR relaxation times (__T__~1ρ~, __T__~2~, and __T__~1~) of water, N‐acetylaspartate (NAA), creatine (Cr), choline‐containing compounds (Cho), and lactate (Lac) were quantified in rat brain at 4.7 T. In control animals, the cerebral __T__~1ρ~ figures, as determined with a spin‐lock

Proton T2 relaxation of cerebral metabol

Proton T2 relaxation of cerebral metabolites during transient global ischemia in rat brain

✍ Hiroyuki Fujimori; Thomas Michaelis; Markus Wick; Jens Frahm 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 382 KB

Putative changes of metabolite T2 relaxation times were investigated before and after a 20-min period of global ischemia in rat brain in vivo (n = 10) using localized proton MRS at different echo times (2.35 T). Neither absolute T2 relaxation times (TE = 20-270 ms) nor time courses of T2-weighted me

Cerebral glucose is detectable by locali

Cerebral glucose is detectable by localized proton NMR spectroscopy in normal rat brain in Vivo

✍ M. L. Gyngell; T. Michaelis; D. Hörstermann; H. Bruhn; W. Hänicke; K. D. Merbold 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 376 KB 👁 1 views

This contribution reports the first direct and noninvasive observation of cerebral glucose in normal anesthetized rats (n = 16) using short-echo-time localized proton NMR spectroscopy (2.35 T, STEAM, TR = 6000 ms, TE = 20 ms, 125 microliters). In addition to resonances from N-acetyl aspartate (NAA),

Cerebral metabolite dynamics during temp

Cerebral metabolite dynamics during temporary complete ischemia in rats monitored by time-shared 1h and 31p nmr spectroscopy

✍ L.-H. Chang; R. Shirane; P. R. Weinstein; T. L. James 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 382 KB

## Abstract The changes in cerebral phosphorus metabolites, intracellular pH, and lactate during 30 min of complete global ischemia and 2 h of reperfusion were monitored by time‐shared ^1^H and ^31^P __in vivo__ NMR spectroscopy in rats. After the induction of ischemia, intracellular pH decreased f

Molecular self-diffusion of intracellula

Molecular self-diffusion of intracellular metabolites in rat brain in vivo Investigated by Localized Proton NMR Diffusion Spectroscopy

✍ Klaus-Dietmar Merboldt; Dirk Hörstermann; Wolfgang Hänicke; Harald Bruhn; Jens F 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 418 KB 👁 1 views

## Abstract Molecular self‐diffusion coefficients of water (0.75 ± 0.05), Nacetylaspartate (0.27 ± 0.04), creatines (0.27 ± 0.04), and cholines (0.28 ± 0.08) × 10^−5^ cm^2^ s^−1^ were obtained from localized proton NMR spectra of rat brain in vivo using diffusion‐weighted stimulated‐echo (STEAM) se

Comparison of lactate concentration dete

Comparison of lactate concentration determinations in ischemic and hypoxic rat brains by in vivo and in vitro1H NMR spectroscopy

✍ L.-H. Chang; B. M. Pereira; P. R. Weinstein; M. A. Keniry; J. Murphy-Boesch; L. 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 395 KB 👁 1 views

An experimental protocol for the quantitation of lactate is demonstrated for in vivo 'H NMR surface coil spectroscopy. The in vivo lactate concentration can be calculated by comparing the in vivo and in vitro ratios of lactate and N-acetylaspartate (NAA) NMR signals. With this protocol, approximatel