𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Comparison of lactate concentration determinations in ischemic and hypoxic rat brains by in vivo and in vitro1H NMR spectroscopy

✍ Scribed by L.-H. Chang; B. M. Pereira; P. R. Weinstein; M. A. Keniry; J. Murphy-Boesch; L. Litt; T. L. James

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
395 KB
Volume
4
Category
Article
ISSN
0740-3194

No coin nor oath required. For personal study only.

✦ Synopsis

An experimental protocol for the quantitation of lactate is demonstrated for in vivo 'H NMR surface coil spectroscopy. The in vivo lactate concentration can be calculated by comparing the in vivo and in vitro ratios of lactate and N-acetylaspartate (NAA) NMR signals. With this protocol, approximately 25% of lactate present in the hypoxic or ischemic rat brain is observed by NMR. @ 1987 Academic press Inc.

📜 SIMILAR VOLUMES

Nonedited 1H NMR lactate/n-acetyl aspart
Nonedited 1H NMR lactate/n-acetyl aspartate ratios and the in vivo determination of lactate concentration in brain
✍ Robert Vink; Tracy K. McIntosh; Alan I. Faden 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 292 KB 👁 1 views

The 1H lactate/n-acetyl aspartate ratio is commonly used for the quantitation of brain lactate under pathological conditions. We demonstrate that the intensity of the lactate methyl region in water-suppressed, nonedited 1H spectra increases following mild brain trauma as a result of factors other th

In vivo1H and 31P NMR spectroscopy of th
In vivo1H and 31P NMR spectroscopy of the developing rat brain
✍ Kazutoshi Hida; Ingrid L. Kwee; Tsutomu Nakada 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 357 KB 👁 1 views

## Abstract The biochemical changes associated with brain maturation during the first 28 days postnatal were investigated utilizing proton and phosphorus‐31 nuclear magnetic resonance spectroscopy in rat pups __in vivo.__ Phosphocreatine was found to increase linearly during this period of developm

in vivo identification and monitoring of
in vivo identification and monitoring of changes in rat brain glucose by two-dimensional shift-correlated 1h nmr spectroscopy
✍ M. Peres; O. Fedeli; B. Barrere; B. Gillet; G. Berenger; J. Seylaz; J-C. Beloeil 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 381 KB 👁 1 views

## Abstract Intracerebral glucose resonance was directly detected and resolved __in vivo__ by two‐dimensional shift‐correlated (COSY) ^1^H NMR spectroscopy in anesthetized rats (__n__ = 4). The relative changes in brain glucose concentration were measured by volume integration of the α‐D‐glucose cr

Metabolism of D- and L-[13C]alanine in r
Metabolism of D- and L-[13C]alanine in rat liver detected by 1H and 13C NMR spectroscopy in vivo and in vitro
✍ Andreas Dölle 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 117 KB 👁 2 views

Alanine is the major amino acid utilized by the liver for gluconeogenesis under normal conditions. The metabolism of alanine in rat liver was investigated by means of 1 H and 13 C NMR spectroscopic studies in vivo and in vitro after infusion of L-and D-alanine labelled with 13 C at the carboxyl and

Detection of [1,6-13C2]-glucose metaboli
Detection of [1,6-13C2]-glucose metabolism in rat brain by in vivo 1H-[13C]-NMR spectroscopy
✍ Robin A. de Graaf; Peter B. Brown; Graeme F. Mason; Douglas L. Rothman; Kevin L. 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 339 KB

## Abstract Localized, water‐suppressed ^1^H‐[^13^C]‐NMR spectroscopy was used to detect ^13^C‐label accumulation in cerebral metabolites following the intravenous infusion of [1,6‐^13^C~2~]‐glucose (Glc). The ^1^H‐[^13^C]‐NMR method, based on adiabatic RF pulses, 3D image‐selected in vivo spectros

Combining CW and pulsed saturation allow
Combining CW and pulsed saturation allows in vivo quantitation of magnetization transfer observed for total creatine by 1H-NMR-spectroscopy of rat brain
✍ Stefan A. Roell; Wolfgang Dreher; Dieter Leibfritz 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 144 KB 👁 2 views

Selective saturation of bound nuclei attenuates the MR visible CH 2 and the CH 3 signal of total creatine (tCr) in rat brain in vivo. The low contrast to noise ratio achieved during the limited experiment time makes it difficult to quantify the effect. It is shown that by combining data from continu