An experimental protocol for the quantitation of lactate is demonstrated for in vivo 'H NMR surface coil spectroscopy. The in vivo lactate concentration can be calculated by comparing the in vivo and in vitro ratios of lactate and N-acetylaspartate (NAA) NMR signals. With this protocol, approximatel
Nonedited 1H NMR lactate/n-acetyl aspartate ratios and the in vivo determination of lactate concentration in brain
β Scribed by Robert Vink; Tracy K. McIntosh; Alan I. Faden
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 292 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0740-3194
No coin nor oath required. For personal study only.
β¦ Synopsis
The 1H lactate/n-acetyl aspartate ratio is commonly used for the quantitation of brain lactate under pathological conditions. We demonstrate that the intensity of the lactate methyl region in water-suppressed, nonedited 1H spectra increases following mild brain trauma as a result of factors other than lactate accumulation.
π SIMILAR VOLUMES
The lactate concentration was estimated from the spectra by comparison to signals from N-acetylaspartate, creatine + phosphocreatine, and water. The effects of Tz, phase modulation, and solvent suppression were taken into account. The estimated concentrations were compared to determinations performe