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Quantitative estimation of lactate in the brain by 1H NMR

โœ Scribed by Stephen R. Williams; Edward Proctor; Kathryn Allen; David G. Gadian; H. Alan Crockard

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
431 KB
Volume
7
Category
Article
ISSN
0740-3194

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โœฆ Synopsis

The lactate concentration was estimated from the spectra by comparison to signals from N-acetylaspartate, creatine + phosphocreatine, and water. The effects of Tz, phase modulation, and solvent suppression were taken into account. The estimated concentrations were compared to determinations performed on the same brains after extraction. The lactate concentration estimated from the intact brain spectra was between 70 and 90% of the values determined in vitro, on the extracts, depending on the concentration standard used. If N-acetylaspartate was used as the standard then the proportion of detected lactate (92%) was not significantly different from 1oc%

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