Cerebral metabolite dynamics during temporary complete ischemia in rats monitored by time-shared 1h and 31p nmr spectroscopy
✍ Scribed by L.-H. Chang; R. Shirane; P. R. Weinstein; T. L. James
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 382 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
The changes in cerebral phosphorus metabolites, intracellular pH, and lactate during 30 min of complete global ischemia and 2 h of reperfusion were monitored by time‐shared ^1^H and ^31^P in vivo NMR spectroscopy in rats. After the induction of ischemia, intracellular pH decreased from 7.14 ± 0.01 to 6.32 ± 0.10, and lactate concentration increased from 1.6 ± 0.4 to 15.8 ± 2.5 μmol/g; ATP and phosphocreatine were totally depleted, while inorganic phosphate increased 715 ± 47%. Within 1 h after blood flow was restored, high‐energy phosphates and lactate levels had recovered close to baseline levels. The changes in intracellular pH and lactate levels during ischemia and reperfusion correlated Well.