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Localization of the luminol-dependent chemiluminescence reaction in human granulocytes

✍ Scribed by Dahlgren, Claes ;Follin, Per ;Johansson, Agneta ;Lock, Ronny ;Orselius, Kristina

Publisher: John Wiley and Sons
Year: 1989
Weight: 294 KB
Volume: 4
Category: Article
ISSN: 0884-3996
DOI: 10.1002/bio.1170040137

No coin nor oath required. For personal study only.

✦ Synopsis

The granulocyte luminol-dependent chemiluminescence (CL) reaction is linked to the enzyme myeloperoxidase reacting with products of the respiratory burst activation. The results presented in this paper, show that the light generated in granulocytes originate both from intracellular and extracellular reactions; however, depending on the stimulus used the one or the other will dominate the activity measured. Furthermore, lysosomal fusion is proposed to be required for the intracellular CL reaction.

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