Influence of different luminols on the characteristics of the chemiluminescence reaction in human neutrophils

✍ Scribed by Lundqvist, Helen ;Kricka, Larry J. ;Stott, Richard A. ;Thorpe, Gary H. G. ;Dahlgren, Claes

• Publisher: John Wiley and Sons
• Year: 1995
• Weight: 567 KB
• Volume: 10
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170100608

✦ Synopsis

In search for a luminol w i t h very high output of light, 20different luminol samples were tested for their ability t o enhance the chemiluminescence reaction in phorbol myristate acetate activated human neutrophils. We found that the majority of luminols tested (17 samples) gave almost thesame light output from neutrophils, and that the major part of the activity was from an intracellular origin. Owing t o the fact that three isoluminol samples were unable t o monitor respiratory burst activity taking place intracellularly, a very l o w level of chemiluminescence was obtained w i t h these samples. Their light output was, however, greatly increased when horseradish peroxidase or myeloperoxidase was added, showing that the light-generating reaction w i t h isoluminol as well as w i t h luminol is peroxidase-dependent.

The fact that isoluminol could also use myeloperoxidase as amplifying peroxidase, suggests that the lack of measurable intracellular activity in the presence of isoluminol is somehow related t o a limited or restricted diffusion of the molecule t o intracellular sites. The isoluminol system constitutes a sensitive system for measuring release of oxygen metabolites from phagocytic cells.