Catalytic behaviour of iron(II)-oxime complexes in the chemiluminescence reaction of luminol with hydrogen peroxide

✍ Scribed by Yun-Xiang Ci; Jian-Ke Tie; Feng-Ji Yao; Zhao-Lan Liu; Sa Lin; Wei-Qing Zheng

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 431 KB
• Volume: 277
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85090-7

✦ Synopsis

Chemiluminescence produced by the catalytic effect of iron(B)-oxime complexes on the oxidation of luminol by hydrogen peroxide was studied. This is a very fast chemiluminescence reaction and the emission spectrum is similar to that of peroxidase-catalysed chemiluminescence. Different kinds of iron(B)-oxime complexes were compared and it was found that the symmetrical dioxlme complex (four nitrogen ligands) has a higher catalytic activity than the asymmetric monooxime complex (two nitrogen and two oxygen ligands). The presence of EDTA can greatly accelerate the chemiluminescence reaction, which was considered to be a result of the formation of a mixed-ligand complex. Other homologues of EDTA were tested and showed different degrees of enhancement. Optimum conditions for the chemiluminescence reactions were examined. Combined with a flow-injection technique, trace amounts of hydrogen peroxide (2 X 10-'-l X low4 MI and, using glucose oxidase, glucose (0.79-160 pg ml-') can be determined by using the iron(B)-dimethylglyoxime complex as a typical catalyst. The detection limit of hydrogen peroxide was 1.3 X lo-* M. The relative standard deviation was 1% for the determination of 2 x lo-' M H,O, (n = 11). The feasibility of utilizing the proposed method for the determination of glucose in human serum was examined.