Luminol-amplified chemiluminescence activity in human monocytes: A comparison with the activity induced in granulocytes

✍ Scribed by Johansson, Agneta ;Dahlgren, Claes

• Publisher: John Wiley and Sons
• Year: 1990
• Weight: 370 KB
• Volume: 5
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170050108

✦ Synopsis

The characteristics of the luminol-amplified chemiluminescence (CL) induced in mononuclear phagocytes interacting with PMA, FMLP or ionomycin were determined. Azide reduced the CL activity by more than 80%. while superoxide dismutase and catalase had minor effect on the monocyte CL response. The sensitivity of the monocyte CL response, t o the addition of extra peroxidase differed depending on the stimulus used. Furthermore, no direct correlation was obtained between the CL response and superoxide anion or hydrogen peroxide production. In comparison with the response in granulocytes, minor quantitative differences were observed. The mechanism for the light-generating reaction, seems t o be the same in both cell types.