Liquid Chromatography–Atmospheric Pressure Electron Capture Dissociation Mass Spectrometry for the Structural Analysis of Peptides and Proteins
✍ Scribed by Robb, Damon B.; Rogalski, Jason C.; Kast, Juergen; Blades, Michael W.
- Book ID
- 126126247
- Publisher
- American Chemical Society
- Year
- 2012
- Tongue
- English
- Weight
- 1018 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0003-2700
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## Abstract High‐performance liquid chromatography (HPLC) coupled to atmospheric pressure chemical ionization (APCI) mass spectrometry was used for the separation and detection of amino acid and peptide enantiomers. With detection limits as low as 250 pg, 25 amino acids enantiomers were baseline re
Capillary liquid chromatography/mass spectrometry (LC/MS) is usually performed using electrospray ionization. Atmospheric pressure chemical ionization (APCI) has not been used since the flow rates involved (1-10 µL/min) are too low to effect ionization with a standard APCI source. Using a 320 µm ؋ 1