This paper describes the use of high pressure liquid chromatography (hplc) for the rapid analysis at high sensitivity of peptide mixtures from proteolytic digestion of proteins. A reversed phase system has been developed which consists of a cc-Bondapak-fatty acid analysis column and an eluant which
High-pressure liquid chromatography of peptides and proteins XI. The use of cationic reagents for the analysis of peptides by high-pressure liquid chromatography
β Scribed by W.S. Hancock; C.A. Bishop; J.E. Battersby; D.R.K. Harding; M.T.W. Hearn
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 601 KB
- Volume
- 168
- Category
- Article
- ISSN
- 1873-3778
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A new high-pressure liquid molecular weight chromatography column was evaluated for its ability to separate proteins and peptides. The column was able to give a linear separation of compounds between 5,000-700,000 M,. Chromatography of posterior pituitary extracts, tumor-associated fetal antigens, a
## Abstract Cardenolides of __Convallaria majalis__ were separated by reverseβphase HPLC on Partisil PXS 10/25 ODS columns using 25% acetonitrile as mobile phase. Periplorhamnoside, convallatoxin and either convallatoxol or convalloside were resolved from glycoside mixtures. Convallatoxin and perip