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The use of high pressure liquid chromatography (hplc) for peptide mapping of proteins IV

✍ Scribed by W.S. Hancock; C.A. Bishop; R.L. Prestidge; M.T.W. Hearn

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 557 KB
Volume: 89
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90742-x

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✦ Synopsis

This paper describes the use of high pressure liquid chromatography (hplc) for the rapid analysis at high sensitivity of peptide mixtures from proteolytic digestion of proteins. A reversed phase system has been developed which consists of a cc-Bondapak-fatty acid analysis column and an eluant which contains a hydrophilic ion-pairing reagent such as phosphoric acid. Examples described in this paper include the elution profile of a thermolysin digestion of 10 pmol of acyl carrier protein, which was resolved into 13 components in 20 min. In addition, the chromatographic system can be used to follow the progress of a digestion and to monitor the purity of the proteolytic enzyme.

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