✦ LIBER ✦
Use of mini-octadecylsilane ultrasphere column in high-pressure liquid chromatography for protein structural studies
✍ Scribed by Ajit S. Bhown; J.Claude Bennett
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 402 KB
- Volume
- 137
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Using a single mini-octadecylsilane (ODS) 5-Frn ultrasphere column (0.46 X 4.5 cm) and linear gradients of different solvents, all the aspects of protein structural analysis have been defined. The effectiveness of the system has been evaluated by separating the (Y and /j chains of hemoglobin and their tryptic peptides. then performing amino acid analysis and, finally, identifying the phenylthiohydantoin derivatives of amino acids,