The Analysis of β-Blockers by Capillary Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometry

✍ Scribed by Kathryn A. Hutton; David E. Games

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 285 KB
• Volume: 11
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19970422)11:7<735::aid-rcm918>3.0.co;2-d

✦ Synopsis

Capillary liquid chromatography/mass spectrometry (LC/MS) is usually performed using electrospray ionization. Atmospheric pressure chemical ionization (APCI) has not been used since the flow rates involved (1-10 µL/min) are too low to effect ionization with a standard APCI source. Using a 320 µm ؋ 15 cm column and an ion-trap mass spectrometer, interfacing for capillary LC/APCI-MS has been successfully carried out using a post column make-up flow. The system was evaluated using a mixture of eight β-blocker drugs in full-scan mode. Good chromatographic fidelity was obtained. Detection limits for timolol and labetalol were determined with selected-and consecutive-reaction monitoring (SRM and CRM). CRM gave the best signal-to-noise traces and detection at the low femtomol level was realized.