The performance of electrospray ionization and atmospheric pressure chemical ionisation (APCI) for the analysis of carotenoids was investigated. On our instrument, APCI produced spectra which were readily interpreted, and the positive-ion mode proved applicable to all of the compounds. The separatio
Analysis of Polyunsaturated Fatty Acids Using High Performance Liquid Chromatography - Atmospheric Pressure Chemical Ionization Mass Spectrometry
✍ Scribed by Řezanka, Tomáš
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 73 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Analysis of polyunsaturated fatty acids using high performance liquid chromatography -atmospheric pressure chemical ionization mass spectrometry is described. The standard fatty acid methyl esters from 16 to 22 carbons were analyzed by LC-MS with APCI. The effect of orifice voltage and total carbon atoms versus number of double bonds in each homologue on the mass spectra is discussed. The correction coefficients for homologues from saturated fatty acids to hexaenoic acid are also mentioned.
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