High Performance Liquid Chromatography/Atmospheric Pressure Chemical Ionization-Mass Spectrometry for the Determination of Carotenoids

✍ Scribed by Philip A. Clarke; Karen A. Barnes; James R. Startin; Frank I. Ibe; Martin J. Shepherd

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 393 KB
• Volume: 10
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(199611)10:14<1781::aid-rcm748>3.0.co;2-d

✦ Synopsis

The performance of electrospray ionization and atmospheric pressure chemical ionisation (APCI) for the analysis of carotenoids was investigated. On our instrument, APCI produced spectra which were readily interpreted, and the positive-ion mode proved applicable to all of the compounds. The separation of nine compounds in under 14 minutes by gradient elution high-performance liquid chiromatography (HPLC) has been demonstrated. The performance characteristics of positive-ion HPLC/APCI-mass spectrometry have been investigated and documented for the determination of nine carotenoids. With the exception of f3-carotene, the precision of the method expressed as coeflident of variation ranged from 6 to 20%. Limits of detection ranged from 0.1 to 1.4 ng/pL dependent upon the analyte. The method was used to confirm the levels of carotenoids in UK Total Diet survey samples.