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Lethal effect of protamine and histone on competent Bacillus subtilis cells

โœ Scribed by Antohi, Stefan ;Popescu, Alexandru

Publisher
Springer
Year
1979
Tongue
English
Weight
444 KB
Volume
170
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

Under experimental conditions of genetic transformation, protamine and total histone were bactericidal for Bacillus subtilis cells. The abilities to cause lethality were very similar for both, either protamine or histone, with no antagonistic effects amongst these natural polycations. With both basic proteins acting simultaneously the enhancement was higher than a summation of the separate lethal effects. Sublethal concentration of protamine added at the beginning of transformation time, produced a strong inhibition of transforming efficiency. The same concentration added later than 10 min from the start of transformation had no inhibitory effect. These facts together with the absence of inhibition by simple pretreatment of DNA alone as well as the cell protection by protamine against lytic activity of lysozyme, suggest a protamine-cell surface interaction which impedes DNA uptake events.

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