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Electron microscopic demonstration of negative surface charges on cytoplasmic membrances of Bacillus subtilis with protamine-ferritin

✍ Scribed by Prof. Dr. F. Mach; P. Bergmann; U. Pfüller; L. Wölfel


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
683 KB
Volume
22
Category
Article
ISSN
0233-111X

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✦ Synopsis


Abstract

The application of a protamine‐ferritin conjugate for labelling of isolated protoplast membranes of Bacillus subtilis S 13/1 is desribed. Contrary to Mycoplasma membranes which could only be labelled on the outer side of the membrane, ferrtin was deposited on both membrane sides as a single layer without cluster formation.