Large-scale isolation of plasmid DNA and purification of λ phage DNA using hydroxylapatite chromatography
✍ Scribed by Thomas R. Johnson; Joseph Ilan
- Book ID
- 107711882
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 604 KB
- Volume
- 132
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
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## Abstract Hydroxyapatite and related stationary phases increasingly play a role in the downstream processing of high‐value biological materials, such as recombinant proteins, therapeutic antibodies and pharmaceutical‐grade plasmid DNA. Chromatographic hydroxyapatite is an inorganic, ceramic mater
The large-scale purification of plasmid DNA was achieved using fast protein liquid chromatography on a Hi-Load Q Sepharose column. This method allows for the purification of plasmids starting from crude plasmid DNA, prepared by a simple alkaline lysis procedure, to pure DNA in less than 5 h. In cont