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Large scale purification of linear plasmid DNA for efficient high throughput cloning

✍ Scribed by Dr. Marjolaine Noirclerc-Savoye; Benoit Gallet; Florent Bernaudat; Thierry Vernet

Book ID
112012253
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
256 KB
Volume
5
Category
Article
ISSN
1860-6768

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## Abstract We set out to investigate if __E. coli__ genotype plays a significant role in host strain selection for optimal processing of plasmid DNA based on both quality and quantity of supercoiling. Firstly 17 __E. coli__ commercial and non‐commercial strains were selected and their available ge