A procedure for large-scale isolation of RNA-free plasmid and phage DNA without the use of RNase
โ Scribed by Ze'ev Lev
- Book ID
- 107714396
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 850 KB
- Volume
- 160
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A procedure for the removal of RNA and RNA fragments from large quantities of pBR322 plasmid DNA without the use of RNase is described. Sephacryl S-300 is employed for the separation of low-molecular-weight RNA from plasmid DNA molecules on the basis of gel filtration. The technique thus circumvents
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