tion and precipitation as originally described by Birn-Many plasmid isolation procedures use strongly alboim and Doly (6). Recent discussions on the BIONET kaline conditions in the initial stages to facilitate lynewsgroup have revealed that several groups as well sis of the host bacteria. We demonst
A procedure for the isolation and purification of plasmid DNA from Rhizobium meliloti
โ Scribed by T. Adachi; V.N. Iyer
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 710 KB
- Volume
- 101
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
A procedure is described for the isolation and purification of the DNA of plasmids that are indigenous to the agriculturally important nitrogen-fixing bacterium Rhizobium meliloti.
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A simple nonenzymatic procedure is described for the separation of DNA, RNA, and proteins of cultured animal cells. DNA isolated by this procedure is large molecular weight and double stranded.