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A Rapid Protocol for the Purification of Plasmid DNA Template Suitable for DNA Sequencing

โœ Scribed by M.K. Majumdar; M. Mcandrewshill; D.A. Williams

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
177 KB
Volume
208
Category
Article
ISSN
0003-2697

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A rapid technique for the determination of unknown plasmid library insert DNA sequence directly from intact yeast cells
โœ Klebanow, Edward R.; Weil, P. Anthony ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 230 KB ๐Ÿ‘ 3 views

A polymerase chain reaction (PCR)-based technique is described which allows for the determination of library plasmid insert DNA sequence directly and rapidly from intact yeast cells. Yeast spheroplasts are used to template a PCR reaction to amplify the insert sequence. This PCR product is then purif