✦ LIBER ✦
Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase
✍ Scribed by Michael V. Norgard
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 859 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A procedure for the removal of RNA and RNA fragments from large quantities of pBR322 plasmid DNA without the use of RNase is described. Sephacryl S-300 is employed for the separation of low-molecular-weight RNA from plasmid DNA molecules on the basis of gel filtration. The technique thus circumvents many of the dangers associated with treating plasmid DNA preparations with RNase. The procedure should be generally applicable to the purification of virtually any type of plasmid DNA isolated from a bacterial host.