An Assay Method for DNA Topoisomerase Ac
โ
Y. Onishi; Y. Azuma; H. Kizaki
๐
Article
๐
1993
๐
Elsevier Science
๐
English
โ 429 KB
A method for assaying the activities of DNA topoisomerases I and II has been developed. The assay for type I and II enzymes is based on monitoring relaxation of supercoiled plasmid DNA in the absence or presence of ATP, respectively. The reaction product, relaxed DNA, was separated from the substrat