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Rapid method for purification of plasmid DNA and DNA fragments from DNA linkers using high-performance liquid chromatography on TSK-PW gel

โœ Scribed by Michael E. Himmel; Peter J. Perna; Michael W. McDonell


Book ID
108336047
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
502 KB
Volume
240
Category
Article
ISSN
1873-3778

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A method for assaying the activities of DNA topoisomerases I and II has been developed. The assay for type I and II enzymes is based on monitoring relaxation of supercoiled plasmid DNA in the absence or presence of ATP, respectively. The reaction product, relaxed DNA, was separated from the substrat