A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extraction is followed by the novel use of tangential flow
✦ LIBER ✦
Purification of plasmid DNA using tangential flow filtration and tandem anion-exchange membrane chromatography
✍ Scribed by Patricia Guerrero-Germán; Duarte M. F. Prazeres; Roberto Guzmán; Rosa Ma. Montesinos-Cisneros; Armando Tejeda-Mansir
- Book ID
- 106146886
- Publisher
- Springer
- Year
- 2008
- Tongue
- English
- Weight
- 347 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1615-7605
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