𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Purification of plasmid DNA using tangential flow filtration and tandem anion-exchange membrane chromatography

✍ Scribed by Patricia Guerrero-Germán; Duarte M. F. Prazeres; Roberto Guzmán; Rosa Ma. Montesinos-Cisneros; Armando Tejeda-Mansir


Book ID
106146886
Publisher
Springer
Year
2008
Tongue
English
Weight
347 KB
Volume
32
Category
Article
ISSN
1615-7605

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Purification of plasmid DNA by tangentia
✍ David W. Kahn; Michelle D. Butler; Darien L. Cohen; Margaret Gordon; Jeanne W. K 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 165 KB 👁 1 views

A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extraction is followed by the novel use of tangential flow

Purification of plasmid DNA by an integr
✍ D. Kendall; G. J. Lye; M. S. Levy 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 176 KB

There is an increasing interest in the development of scaleable and reproducible plasmid DNA purification protocols for vaccine and gene therapy. The use of an integrated unit operation, comprising tangential flow microfiltration coupled with the adsorption of contaminants onto nitrocellulose membra