Kinetic analysis of the effect of cell density on hybridoma cell growth in batch culture
β Scribed by Eun Yeol Lee
- Publisher
- The Korean Society for Biotechnology and Bioengineering
- Year
- 2002
- Tongue
- English
- Weight
- 290 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1226-8372
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The cell growth and monoclonal antibody production kinetics of hybridoma cell cultures continuously exposed to growth factors and the cognate antigen were investigated. The growth factors were the epidermal growth factor, fibroblast growth factor, and interleukin-2, whereas the antigen was the trini
Hybridoma fed-batch cultures with either standard medium as feed or concentrated medium as feed and removal of toxic metabolites through dialysis were performed by using model calculations for "a priori" determination of process parameters. In a first step a kinetic model for specific growth