Growth and product inhibition kinetics of T-cell hybridomas producing lymphokines in batch and continuous culture
β Scribed by T.S. Shaughnessy; F. Kargi
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 564 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0141-0229
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A hybridoma cell line, AFP-27-P, was cultivated in continuous culture under glucose-limited conditions. The viable cell concentration, dead-cell concentration, and cell volume all varied with the dilution rate. A model previously developed for a nonproducing clone of the same cell line, AFP-27-NP, w
## Abstract The kinetic behavior of a nonproducing hybridoma clone AFPβ27βNP was investigated in continuous culture under glucoseβlimited conditions. A total of more than 21, 000 h of cultures were operated at dilution rates ranging from 0.01 to 0.06 h^β1^. The viable cell concentrations, dead cell