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Karyology and tumorigenicity of a Simian virus 40-transformed chinese hamster cell clone

✍ Scribed by John M. Lehman; Katrina Trevor

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
522 KB
Volume
98
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

A pseudodiploid clone of Chinese hamster cells transformed in vitro with Simian virus 40 (SV~40~) was isolated from soft agar and injected into nude mice through three successive passages with a short in vitro cultivation between each animal inoculation. The original clone and the three subsequent tumor populations were characterized in regard to SV~40~ T antigen staining, modal chromosome number, and Giemsa‐banded karyotype. All tumor cell lines maintained the pseudodiploid mode, as well as the positive SV~40~ T antigen staining. Nonrandom chromosomal changes included loss of one of the X chromosomes, additions of abnormal variants of chromosomes No. 1 and No. 2, the appearance of unidentified marker chromosomes, and the loss of autosomes No. 5, No. 6, and No. 11. The deletion of one of the X chromosomes occurred with about the same frequency in all cell lines. Additions of abnormal chromosomes No. 1 and No. 2 tended to recur more often in the tumor cell lines than in the original clone. The appearance of marker chromosomes, as well as the loss of autosomes No. 5, No. 6, and No. 11 demonstrated a correlation with tumorigenicity. Yet, the three successive passages of the cells through the animal did not select for a tumor population with a single, homogeneous karyotype.

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