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Flow microfluorometry analysis of DNA content in chinese hamster cells following infection with simian virus 40

✍ Scribed by P. K. Horan; J. H. Jett; A. Romero; J. M. Lehman

Publisher: John Wiley and Sons
Year: 1974
Tongue: French
Weight: 496 KB
Volume: 14
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910140411

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✦ Synopsis

Abstract

Infection of secondary Chinese hamster cells with SV40 virus induces a population of cells into consecutive S periods. These changes in DNA content were examined at various times post infection by means of flow microfluorometry (FMF) which enabled a kinetic analysis to be made. At 12 h post infection, a population of 6C cells was observed which was induced from late S or G~2~ cells. By 24 h post infection, cells at 8C, 10C, 12C, 16C, and intermediate DNA contents were detected. The induction of this population of cells occurred within the first 24 h post infection. At later time intervals, there was a decrease in total number of cells with DNA contents of 8C or 16C.

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