Early chromosome changes in diploid Chinese hamster cells after infection with simian virus 40

✍ Scribed by John M. Lehman

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: French
• Weight: 483 KB
• Volume: 13
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910130203

✦ Synopsis

Abstract

Primary and secondary Chinese hamster (ChH) embryo cells infected with SV40 exhibited chromosomal changes within one cell generation (24 h). The initial change seen is an increase in the number of polyploid metaphases (>10%). The majority of the polyploid cells are tetraploid (8×), however, higher ploidy values were also observed (16 ×, 32 × and 64 ×). In approximately 20–30% of the polyploid metaphases there were chromosome changes such as breaks, abnormal chromosomes and loss and addition of chromosomes. The remaining polyploids were normal in regard to chromosome number and chromosome morphology. A primary mouse embryo cell system also responded to SV40 infection with an increase in the number of polyploid cells within 48 h.