Tumorigenicity of tsA and wild-type simian virus 40 transformed cells inoculated onto the chicken chorioallantoic membrane
✍ Scribed by John M. Lehman; Patricia Estes
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- French
- Weight
- 728 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
This study compares the tumorigenicity of SV40 primary tumor cell lines, tsA and wild‐type SV40‐transformed Chinese hamster cells, at two temperatures, 37° C and 40.5° C, inoculated onto the chorioallantoic membrane of the chicken egg. The SV40 primary tumor cell lines varied in their efficiency of takes at 37° C from 78% for the H65–90B tumor line, 73% for the H80–7A and 25% for the H80‐4 line. At 40.5° C the H80‐4 was unable to form tumors; however, the H80–7A and H80‐4 produced 70% and 20% tumors respectively. Histologically, all CAM tumors were fibrosarcomas identical to the transplanted tumors, however, the tumor(s) at 40.5° C were smaller. Chinese hamster wild‐type SV40‐transformed cells were equally efficient (32%) at tumor production at both temperatures. The tsA‐SV40‐transformed Chinese hamster cells (A58 and A58–2) induced 34% tumors at 37° C and 9% tumors at 40.5° C. At 37° C these tumors were typical fibrosarcomas; however, the 40.5° C tumors were smaller and less cellular, resembling a more differentiated fibrosarcoma. Therefore, the tsA Chinese hamster transformed cells were less efficient at tumor induction at the non‐permissive temperature; however, the primary tumor lines also demonstrated a variability in tumorigenicity (H65–90B and H80–4). Possibly factors other than the temperature‐sensitive viral protein (big “T” antigen) may be involved in establishing a tumor on the chicken CAM.