Tumorigenicity and agglutination by concanavalin a of Chinese hamster cells and their hybrids

✍ Scribed by Ph. de Micco; Monique Berebbi

• Publisher: John Wiley and Sons
• Year: 1972
• Tongue: French
• Weight: 334 KB
• Volume: 10
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910100204

✦ Synopsis

Abstract

In a previous work we studied the relationship between tumorigenicity and the karyological data of a somatic hybrid derived from two lines of Chinese hamster cells, of which one was tumorigenic and the other had lost its tumorigenicity. In this work, we show the relationship between the two factors previously studied and the agglutinating ability of these cells by Concanavalin A. In order to do this, we first derived a coefficient α to express quantitatively the agglutinating ability with Concanavalin A. This coefficient was a function of the latency period, the time necessary to reach the platform level of agglutinating ability and the degree of agglutination of the cells in presence of a fixed quantity of cells and Concanavalin A. The highly tumorigenic DC‐3F cell line gave a high a value, the non‐tumorigenic DC‐3F/ADX/Aza had a much lower value and the overall hybrid population (HyC) had an intermediary value for α. The clones and the tumors studied show a correlation between the value and the tumorigenicity of the cells involved. The karyological analysis showed, inter alia, a possible correlation between the presence of a marker M2 and a particularly low a coefficient.