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Agglutination of normal and neoplastic human cells by concanavalin A and ricinus communis agglutinin

✍ Scribed by Bengt Glimelius; Bengt Westermark; Jan Pontén

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
850 KB
Volume
14
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Agglutination by two lectins, Con A and to a lesser extent ricin, has been investigated in human glia and glioma cells serially cultivated in vitro. No significant difference was found between the normal and transformed cells. The latter formed a spectrum where agglutination could be below or above the level of the control glia lines. Neither was there any difference between normal adult skin fibroblasts and two human sarcoma lines. We suggest that strictly normal cells in vitro are agglutinable by lectins such as Con A. This is also the case for most spontaneous, virus‐ or chemically transformed cells. We believe that exceptions to this rule—exemplified by lines such as 3T3, BHK and FE (a feline embryo line)—have acquired a membrane abnormality responsible for their low degree of agglutinability.

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