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Formation of extracellular adenosine triphosphate by normal and neoplastic human cells in culture

✍ Scribed by G. Ågren; J. Ponten; G. Ronquist; B. Westermark

Publisher
John Wiley and Sons
Year
1971
Tongue
English
Weight
418 KB
Volume
77
Category
Article
ISSN
0021-9541

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✦ Synopsis

Normal and neoplastic human cells in culture were suspended under isotonic conditions and incubated for one minute with the substrates, including 82P-labelled inorganic phosphate, and cofactors of the glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase reactions (complete medium), as well as with an incomplete medium lacking ADP, NAD+ and glyceraldehyde-3-phosphoric acid. The neoplastic cell types incubated in the complete medium synthesized three to six times more labelled ATP than the corresponding normal cells. In the incomplete medium only insignificant amounts of labelled

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