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Agglutination by concanavalin A of normal chick embryo fibroblasts treated by 5-bromodeoxyuridine (BrdU)

✍ Scribed by Jean-Michel Biquard

Publisher: John Wiley and Sons
Year: 1974
Tongue: English
Weight: 354 KB
Volume: 84
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040840313

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✦ Synopsis

Abstract

When chick embryo fibroblasts are grown for two days in the presence of low doses (18 μg/ml) of 5‐bromodeoxyuridine (BrdU), their agglu‐tinability by Concanavalin A is increased to about the same degree as following viral transformation of the cells by Rous sarcoma virus. Simultaneous addition of a large excess, but not of a low dose of thymidine prevents this effect of BrdU.

Treatment with BrdU also enhances the agglutinability of fibroblasts infected with a conditional It mutant of Rcus sarcoma virus at the temperature of 41° which restricts morphological transformation.

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## Abstract The rescue of RSV from the “poorly” virogenic RSV‐transformed rat lines LW13‐RsK1 and LW13‐RsK4 was achieved only after treatment with 5‐bromodeoxyuridine (BUdR) before fusion with chicken embryo fibroblasts. Treatment of these lines with mitomycin C (MC) before fusion did not result in