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Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae

✍ Scribed by Rosenbluh, Amy ;Mevarech, M. ;Koltin, Yigal ;Gorman, Jessica A.

Publisher: Springer
Year: 1985
Tongue: English
Weight: 285 KB
Volume: 200
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425739

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✦ Synopsis

A genomic library of the asexual pathogenic yeast Candida albicans was constructed in the S. eerevisiae vector YEp13. The library contains a representation of the entire genome with a probability of 99%. The expression of the genes of C. albicans in S. cerevisiae was examined and two mutations his3-1 and trp1-289 of S. cerevisiae were complemented by the cloned genes of C. albicans. The hybridization data indicates that the plasmids complementing the mutations of S. cerevisiae contain sequences from C.

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