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Isolation and sequence of the gene encoding ornithine decarboxylase,SPE1, fromCandida albicans by complementation of aspe1Δ ctrain of Saccharomyces cerevisiae

✍ Scribed by Mcnemar, Mark D.; Gorman, Jessica A.; Buckley, Helen R.

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 154 KB
Volume: 13
Category: Article
ISSN: 0749-503X
DOI: 10.1002/(sici)1097-0061(199711)13:14<1383::aid-yea189>3.0.co;2-m

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✦ Synopsis

The gene encoding ornithine decarboxylase, SPE1, from the pathogenic yeast Candida albicans has been isolated by complementation of an ornithine decarboxylase-negative (spe1 delta) strain of Saccharomyces cerevisiae. Four transformants, three of which contain plasmids with the SPE1 gene, were isolated by selection on polyamine-free medium. The C. albicans ornithine decarboxylase (ODC) showed high homology with other eukaryotic ODCs at both the amino acid and nucleic acid levels.

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