A genomic library of the asexual pathogenic yeast Candida albicans was constructed in the S. eerevisiae vector YEp13. The library contains a representation of the entire genome with a probability of 99%. The expression of the genes of C. albicans in S. cerevisiae was examined and two mutations his3-
โฆ LIBER โฆ
Isolation of Hem3 mutants from Candida albicans by sequential gene disruption
โ Scribed by Kurtz, Myra B. ;Marrinan, Jean
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 646 KB
- Volume
- 217
- Category
- Article
- ISSN
- 0026-8925
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A gene bank of Sau3A partially digested Candida albicans DNA in vector YEp13 was used to complement a ura3 mutation (orotidine-5'-phosphate decarboxylase, OMPdecase) in S. cerevisiae. Two plasmids which complemented ura3 and showed clear linkage of Ura+ and plasmid markers were selected for further