Isolation of the catalase A gene of Saccharomyces cerevisiae by complementation of the cta1 mutation

✍ Scribed by Cohen, Gerald ;Fessl, Friederike ;Traczyk, Aleksandra ;Rytka, Joanna ;Ruis, Helmut

• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 935 KB
• Volume: 200
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00383315

✦ Synopsis

As a first step in an analysis of the DNA regions involved in the control of the catalase A gene of Saecharomyees cerevisiae by glucose, heine, and oxygen this gene has been cloned. Catalase A-deficient mutants were obtained by UV mutagenesis of a cttl mutant strain specifically lacking catalase T. All the catalase A-deficient mutants obtained fall into one complementation group. The single recessive mutation causing specific lack of catalase A was designated ctal. Several overlapping DNA fragments complementing the ctal mutation were obtained by transforming cttl ctal double mutants with a yeast gene library in vector YEpl3. Hybrid selection of RNA with the help of one of the cloned DNAs followed by in vitro translation of this RNA and identification of the protein synthesized with catalase A-specific antibodies showed that the catalase A structural gene has been cloned. A single copy of this gene is present in the yeast genome. Transcription of the catalase A gene cloned into vector YEpI3 is repressed by glucose. The DNA isolated hybridizes to a 1.6 kb polyA +-RNA virtually absent from heme-deficient cells, presumably catalase A mRNA,