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Isolation and purification of oridonin from Rabdosia rubescens using upright counter-current chromatography

✍ Scribed by Yanbin Lu; Cuirong Sun; Yuanjiang Pan

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
363 KB
Volume
29
Category
Article
ISSN
1615-9306

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✦ Synopsis

Isolation and purification of oridonin from Rabdosia rubescens using upright counter-current chromatography

A preparative counter-current chromatography (CCC) method for isolation and purification of oridonin, a new cancer chemoprevention agent, from the Chinese medicinal plant Rabdosia rubescens was successfully established. The crude oridonin was obtained by elution with a light petroleum/acetone solvent mixture from ethanol extracts of R. rubescens using column chromatography on silica gel. With a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (1 : 2 : 1 : 2, v/v), 120 mg of oridonin at the purity of 97.8% was obtained from 200 mg of the crude sample in a single-step CCC separation. The structure of oridonin was identified by ESI-MS, 1 H NMR, and 13 C NMR.

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