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Preparative isolation and purification of two amides from Mallotus lianus Croiz by high-speed counter-current chromatography

✍ Scribed by Liyan Jiang; Yanbin Lu; Shan He; Yuanjiang Pan; Cuirong Sun; Tianxing Wu

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
457 KB
Volume
31
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

High‐speed counter‐current chromatography (HSCCC) was applied to the preparative isolation and purification of two amides from Mallotus lianus Croiz. In a single HSCCC separation, using the two‐phase solvent system composed of n‐hexane/ethyl acetate/methanol/water (5:1:5:1 v/v), 247.5 mg of the enriched crude sample was separated to afford 10.3 mg of N‐isobutyl‐2__E__,4__E__,12__Z__‐octadecatrienamide and 15.7 mg of (7__Z__,10__Z__,18__Z__)tricosa‐7,10,18‐trienamide, a novel compound, with the purities of 98.0 and 94.6%, respectively. The HSCCC fractions were analyzed by HPLC and chemical structures of the compounds were identified by 1D‐ and 2D‐NMR, ESI‐, and GC‐MS.

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