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Preparative isolation and purification of atractylon and atractylenolide III from the Chinese medicinal plant Atractylodes macrocephala by high-speed counter-current chromatography

✍ Scribed by Chunxia Zhao; Chaohong He

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
456 KB
Volume
29
Category
Article
ISSN
1615-9306

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✦ Synopsis

Preparative isolation and purification of atractylon and atractylenolide III from the Chinese medicinal plant Atractylodes macrocephala by high-speed counter-current chromatography

The medicinal plant Atractylodes macrocephala (Baizhu in Chinese) has been widely used in traditional Chinese medicine for energy and stomach complaints, treatment of dyspepsia and anorexia, anti-inflammation, anticancer and for increasing assimilation. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of two main bioactive components, namely, atractylon and atractylenolide III from A. macrocephala by using light petroleum (60 -908C) -ethyl acetate -ethanol -water (4 : 1 : 4 : 1 v/v) as the two-phase solvent system in dual-mode elution. Compared with the separation using the normal-mode elution, the dual-mode HSCCC can be achieved with shorter elution time. Atractylenolide III (32.1 mg) at 99.0% purity and 319.6 mg atractylon at 97.8% purity could be obtained from 1000 mg crude sample in a single run. The recoveries of atractylenolide III and atractylon were 95.4 and 92.6%, respectively.

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