Preparative isolation of flavonoid compounds from Oroxylum indicum by high-speed counter-current chromatography by using ionic liquids as the modifier of two-phase solvent system

✍ Scribed by Renmin Liu; Lili Xu; Aifeng Li; Ailing Sun

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 226 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900612

✦ Synopsis

Abstract

A preparative high‐speed counter‐current chromatography method for isolation and purification of flavonoid compounds from Oroxylum indicum was successfully established by using ionic liquids as the modifier of the two‐phase solvent system. Two flavonoid compounds including baicalein‐7‐O‐diglucoside and baicalein‐7‐O‐glucoside were purified from the crude extract of O. indicum by using ethyl acetate–water–[C~4~mim][PF~6~] (5:5:0.2, v/v) as two‐phase solvent system. 36.4 mg of baicalein‐7‐O‐diglucoside and 60.5 mg of baicalein‐7‐O‐glucoside were obtained from 120 mg of the crude extract. Their purities were 98.7 and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by ^1^H‐NMR and ^13^C‐NMR.