𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Isolation and purification of canthaxanthin from the microalga Chlorella zofingiensis by high-speed counter-current chromatography

✍ Scribed by Hua-Bin Li; King-Wai Fan; Feng Chen

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
563 KB
Volume
29
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

✦ Synopsis

Isolation and purification of canthaxanthin from the microalga Chlorella zofingiensis by high-speed counter-current chromatography Certain microalgae are considered to be a potential source of canthaxanthin, which possesses strong antioxidant and anticancer activities. A high-speed counter-current chromatography (HSCCC) method was developed for the separation and purification of canthaxanthin from the microalga Chlorella zofingiensis. The crude canthaxanthin was obtained by extraction with organic solvents after the microalgal sample had been saponified. Preparative HSCCC, with a two-phase solvent system composed of n-hexane-ethanol-water (10:9:1 v/v), was successfully performed yielding canthaxanthin at 98.7% purity from 150 mg of the crude extract (2.1% canthaxanthin) in a one-step separation. The recovery of canthaxanthin was 92.3%. This was the first report that canthaxanthin was successfully separated and purified from microalgae.

📜 SIMILAR VOLUMES

Isolation and purification of macrocycli
Isolation and purification of macrocyclic components from Penicillium fermentation broth by high-speed counter-current chromatography
✍ Xiang Gao; Rongqiang Zhuang; Jiannan Guo; Jian Bao; Meijuan Fang; Yan Liu; Pengx 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 348 KB

## Abstract In this paper, high‐speed counter‐current chromatography (HSCCC), assisted with ESI‐MS, was first successfully applied to the preparative separation of three macrolide antibiotics, brefeldin A (12.6 mg, 99.0%), 7′‐__O__‐formylbrefeldin A (6.5 mg, 95.0%) and 7′‐__O__‐acetylbrefeldin A (5

Preparative isolation and purification o
Preparative isolation and purification of anthocyanins from purple sweet potato by high-speed counter-current chromatography
✍ Fan Qiu; Jianguang Luo; Shun Yao; Li Ma; Lingyi Kong 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 670 KB

## Abstract High‐speed counter‐current chromatography (HSCCC) was applied to the preparative isolation and purification of peonidin 3‐__O__‐(6‐__O__‐(__E__)‐caffeoyl‐2‐__O__‐β‐D‐glucopyranosyl‐β‐D‐glucopyranoside)‐5‐__O__‐β‐D‐glucoside (**1**), cyanidin 3‐__O__‐(6‐__O__‐__p__‐coumaroyl)‐β‐D‐glucopy

Preparative isolation and purification o
Preparative isolation and purification of two amides from Mallotus lianus Croiz by high-speed counter-current chromatography
✍ Liyan Jiang; Yanbin Lu; Shan He; Yuanjiang Pan; Cuirong Sun; Tianxing Wu 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 457 KB

## Abstract High‐speed counter‐current chromatography (HSCCC) was applied to the preparative isolation and purification of two amides from __Mallotus lianus__ Croiz. In a single HSCCC separation, using the two‐phase solvent system composed of __n__‐hexane/ethyl acetate/methanol/water (5:1:5:1 v/v),

Preparative isolation and purification o
Preparative isolation and purification of antioxidative diarylheptanoid derivatives from Alnus japonica by high-speed counter-current chromatography
✍ Soon Sung Lim; Min Young Lee; Hong Ryul Ahn; Soon Jung Choi; Jae-Yong Lee; Sang 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 228 KB

## Abstract This study employed the online HPLC‐2,2′‐azino‐__bis__(3‐ethylbenzthiazoline‐6‐sulphonic acid) (ABTS)^+^ bioassay to rapidly determine the antioxidant compounds occurring in the crude extract of __Alnus japonica__. The negative peaks of the ABTS^+^ radical scavenging detection system, w