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Preparative isolation and purification of senkyunolide-I, senkyunolide-H and ferulic acid from Rhizoma Chuanxiong using counter-current chromatography

✍ Scribed by Yun Wei; Jia Hu; Hao Li; Jiangang Liu

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
185 KB
Volume
34
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Three active compounds, senkyunolide‐I, senkyunolide‐H and ferulic acid (FA), were successfully isolated and purified from the extracts of Rhizoma Chuanxiong by counter‐current chromatography (CCC). Based on the principle of the partition coefficient values (k) for target compounds and the separation factor (α) between target compounds, the two‐phase solvent system that contains n‐hexane–ethyl acetate–methanol–water at an optimized volume ratio of 3:7:4:6 v/v was selected for the CCC separation, and the lower phase was employed as the mobile phase in the head‐to‐tail elution mode. In a single run, 400 mg of the crude extract yielded pure senkyunolide‐I (6.4 mg), senkyunolide‐H (1.7 mg) and FA (4.4 mg) with the purities of 98, 93 and 99%, respectively. The CCC fractions were analyzed by high‐performance liquid chromatography, and the structures of the three active compounds were identified by MS and ^1^H NMR.

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