Preparative isolation of pseudolaric acids A and B, and their glucosides from the root bark of Pseudolarix kaempferi using high-speed counter-current chromatography

✍ Scribed by Quan-Bin Han; Lina Wong; Fanny Lai; Nian-Yun Yang; Jing-Zheng Song; Chun-Feng Qiao; Hong-Xi Xu

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 405 KB
• Volume: 32
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200800547

✦ Synopsis

Abstract

In order to provide the chemical markers for the quality control of herbal medicines, four diterpenoids, pseudolaric acids A and B (PAA and PAB), and their glucosides were isolated from the methanol extract of the Chinese herb Pseudolarix kaempferi using high‐speed counter‐current chromatography (HSCCC). The diphase solvent system was n‐hexane/EtOAc/MeOH/H~2~O which was used at two ratios (5:5:5:5 and 1:9:4:6 by volume) in the separation of pseudolaric acids and their glycosides, respectively. As a result, PAA (14 mg), PAB (129 mg), PAA‐O‐β‐D‐glucopyranoside (8 mg, PAAG), and PAB‐O‐β‐D‐glucopyranoside (42 mg, PABG) were obtained from 0.5 g of the crude extract. Their purities were determined to be above 97% by HPLC analysis. Their chemical structures were confirmed by ^1^H and ^13^C NMR analysis or HPLC comparison with the reference compounds.