Preparative isolation and purification of anti-tumor agent ansamitocin P-3 from fermentation broth of Actinosynnema pretiosum using high-performance counter-current chromatography
✍ Scribed by Yuqin Yao; Zhihui Cheng; Haoyu Ye; Yongmei Xie; Jing He; Minghai Tang; Tao Shen; Jiangman Wang; Yan Zhou; Zejun Lu; Feng Luo; Lijuan Chen; Luoting Yu; Jin-Liang Yang; Aihua Peng; Yuquan Wei
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 243 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
Ansamitocin P‐3 is a potent anti‐tumor maytansinoid found in Actinosynnema pretiosum. However, due to the complexity of the fermentation broth of Actinomycete, how to effectively separate ansamitocin P‐3 is still a challenge. In this study, both analytical and preparative high‐performance counter‐current chromatography were successfully used to separate and purify ansamitocin P‐3 from fermentation broth. A total of 28.8 mg ansamitocin P‐3 with purity of 98.4% was separated from 160 mg crude sample of fermentation broth in less than 80 min with the two‐phase solvent system of hexane–ethyl acetate–methanol–water (0.6:1:0.6:1, v/v/v/v). The purity and structural identification were determined by HPLC, ^1^H NMR, ^13^C NMR and mass spectroscopy.