✦ LIBER ✦

A comparative study of upright counter-current chromatography and high-performance liquid chromatography for preparative isolation and purification of phenolic compounds from Magnoliae officinalis

✍ Scribed by Yanbin Lu; Cuirong Sun; Yuanjiang Pan

Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 541 KB
Volume: 29
Category: Article
ISSN: 1615-9306
DOI: 10.1002/jssc.200500363

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✦ Synopsis

A comparative study of upright counter-current chromatography and high-performance liquid chromatography for preparative isolation and purification of phenolic compounds from Magnoliae officinalis A comparative study of preparative isolation and purification of the phenolic compounds magnolol and honokiol from the Chinese medicinal plant Magnoliae officinalis by upright counter-current chromatography (CCC) and semi-preparative HPLC is presented. The comparison reveals that with a two-phase solvent system composed of light petroleum (bp 60 -908C) -ethyl acetate -tetrachloromethane -methanolwater (1:1:8:6:1, v/v), 1250 mg of honokiol and 520 mg of magnolol, with a purity of 98.7 and 99.5%, respectively, were obtained from 2.0 g of a crude sample of Magnoliae officinalis in a single CCC separation. In contrast, semi-preparative HPLC allowed isolation and purification of these two phenolic compounds with significantly lower productivity and higher solvent consumption. Structures of the purified compounds were identified by 1 H and 13 C NMR.

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